Rhobust™ > Ligand Chemistry

Mixed Mode Ligand Chemistry

Mixed Mode ligands combine the effects of hydrophobic and ionic interactions in a unique pH dependent manner. Typically Mixed Mode ligands bind the target molecule even at high ionic strength and release it again by a simple change of pH. This is particularly relevant in industrial applications where the crude raw material often contains relatively high salt concentrations. Upfront has a range of proprietary mixed mode binding ligands that mediate the interaction between adsorbent and target biomolecules. These ligands capture the target biomolecules directly from the raw material providing a high degree of selectivity.

Mixed mode ligands can be chosen to fit optimal process operations. It is possible to control the capture and release of target molecules by simple changes of pH, which can be readily implemented in large-scale production facilities and maintain biological activity of the product.

Ligands in Upfront's library are selected for their suitability for industrial processing, in particular their stability to 1M NaOH, low toxicity, and very low leakage from adsorbent. The technology offers operational robustness and versatility for both primary capture and fine purification of a broad range of biomolecules and functional proteins.

Ligand Chemistry | High Specificity | Robustness